Investigation of end processing and degradation of premature tRNAs and their application to stabilization of in vitro transcripts in wheat germ extract.
نویسندگان
چکیده
We investigated the end processing and degradation of premature tRNAs in wheat germ extract (WGE), which led to the discovery of end protectors useful for stabilizing an in vitro transcript against various ribonucleases and thereby enhancing its apparent activity in WGE.
منابع مشابه
A comparative study on the effects of wheat germ extract and flexibility exercises on primary dysmenorrhea in non-athlete female students: A randomized clinical trial
Introduction: Primary dysmenorrhea is usually one of the most common complaints of women in childbearing age that affects their quality of life and their social activity. Various methods have been mentioned to control and treat dysmenorrhea. Therefore, the aim of this study was to compare the wheat germ extract intake and flexibility exercises on primary dysmenorrhea in non-athlete female stude...
متن کاملProtective Effects of Wheat Germ Oil on the IVF, Oxidative Stress and In Vitro Zygote Development in Carbamazepine Treated Epileptic Male Mice
Abstract Background and Objectives: Epilepsy is a neurological disorder caused by abnormal electrical discharges from the brain. Carbamazepine is used to treat epilepsy. Over time, this drug may increase oxidative stress and reproductive abnormalities in patients with epilepsy. Wheat germ oil has the highest amount of vitamin E, among other vegetable oils. The aim of this study was to evaluate ...
متن کاملProcessing of transfer RNA precursors in a wheat mitochondrial extract.
To investigate mechanisms for processing of plant mitochondrial RNAs, we studied the fate of wheat mitochondrial tRNA precursors in a homologous soluble extract. Artificial precursor transcripts were synthesized in vitro using T3 or T7 RNA polymerase and DNA templates containing wheat mitochondrial tRNA genes and flanking sequences. We found that the mitochondrial extract supports processing of...
متن کاملThe Effect of Wheat Germ Extract on Premenstrual Syndrome Symptoms
Pre-menstrual syndrome is one of the most common disorders in women and causes impairment in work and social relationships. Several treatment modalities have been proposed, one of which is herbal medicines. Considering the properties of wheat germ, this study aimed to determine the effects of wheat germ extract on the symptoms of premenstrual syndrome.This triple blind clinical trial was conduc...
متن کاملThe Effect of Wheat Germ Extract on Premenstrual Syndrome Symptoms
Pre-menstrual syndrome is one of the most common disorders in women and causes impairment in work and social relationships. Several treatment modalities have been proposed, one of which is herbal medicines. Considering the properties of wheat germ, this study aimed to determine the effects of wheat germ extract on the symptoms of premenstrual syndrome.This triple blind clinical trial was conduc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Organic & biomolecular chemistry
دوره 13 4 شماره
صفحات -
تاریخ انتشار 2015